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URL: http://www.addgene.org/234816
Proper Citation: RRID:Addgene_234816
Insert Name: gRNA targeting SbLw
Organism: Foxtail Mosaic Virus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:39661735
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Comments: The forward oligo included SapI restriction sites, the duplicated coat protein promoter sequence, and 20-bp sgRNA sequences flanked by the 5' sequences of the sgRNA scaffold. A common reverse oligo (esgRNA in FoMV R) was used to amplify the 3' region of the sgRNA scaffold, including a SapI restriction site for subsequent cloning.
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Source: Addgene