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URL: http://www.addgene.org/228494
Proper Citation: RRID:Addgene_228494
Insert Name: pol
Organism: Synthetic
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Size:5630; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Bacterial expression of 10xHis-SUMO tagged Pfu polymerase fused to Sso7d processivity domain (commercially sold as Phusion Polymerase). Polymerase domain contains the A408S amino acid substitution for enhanced fidelity. Kennedy EM, Hergott C, Dewhurst S, Kim B. The mechanistic architecture of thermostable Pyrococcus furiosus family B DNA polymerase motif A and its interaction with the dNTP substrate. Biochemistry. 2009 Dec 1;48(47):11161-8. doi: 10.1021/bi9010122. PMID: 19817489; PMCID: PMC3097049.
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Source: Addgene
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