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URL: http://www.addgene.org/22841
Proper Citation: RRID:Addgene_22841
Insert Name: A DNA (with MCS)
Organism: Cotton Leaf Crumple Virus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18621976
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript SK+ II (original backbone); Vector Types:Bacterial Expression, cotton VIGS vector; Bacterial Resistance:Ampicillin
Comments: Please note that the actual sequence is slightly different than the map below (which is based on the author's sequence). A T->C mutation eliminates the NruI site. --Must be bombarded with appropriate B DNA for systemic infection
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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