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URL: http://www.addgene.org/220748
Proper Citation: RRID:Addgene_220748
Insert Name: NIR-FbLAG30
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:39242569
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5410; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Comments: GenBank: MW627294.1 (part of the insert). To make pET-NIR-FbLAG30, NIR-FbLAG30 was amplified and inserted to pET22b(+) using NdeI/BamHI restriction enzymes. For cytoplasmic expression pelB signaling sequence (AAATACCTG...ATGGCCATG) was deleted by PCR with a forward primer.
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Source: Addgene
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