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URL: http://www.addgene.org/220740
Proper Citation: RRID:Addgene_220740
Insert Name: NIR-FbLAG30
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:39242569
Vector Backbone Description: Backbone Marker:Invitrogen/Thermo Fisher Scientific; Backbone Size:5045; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Comments: GenBank: MW627294.1 (part of the insert). To make pNIR-FbLAG30, LAG30 nanobody was amplified from pET21-pelB-LAG-30 (Addgene #172754) and then internally fused to miRFP670nano3 amplified from pcDNA-miRFP670nano3 (Addgene #184664). Construct was inserted to pcDNA3.1 plasmid using KpnI and EcoRI restriction enzymes.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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