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URL: http://www.addgene.org/220253
Proper Citation: RRID:Addgene_220253
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:38911550
Vector Backbone Description: Backbone Size:4047; Vector Backbone:pSpy3C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white. Resource Information: To create pSpy3C, we took the minimal pWV01* replicon from pBAV1K-T5-gfp (Plasmid #26702), the chloramphenicol resistance cassette from pIB166 (Plasmid #90189) and the MCS featuring the Plac_mrfp cassette from pBS3Clux (#55172). Please download the file linked below to obtain a well annotated map of the plasmid.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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