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URL: http://www.addgene.org/217310
Proper Citation: RRID:Addgene_217310
Insert Name: Acetamidase
Organism: Aspergillus nidulans
Bacterial Resistance: Ampicillin
Defining Citation: PMID:39194893
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSDMA57; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Contains the mNeonGreen amdS2 fusion reporter in the multicloning site of pSDMA57 which is a modified pBluescript II -SK backbone vector. The vector is designed integration into the Cryptococcus neoformans genome via homologous recombination with the Safe Haven site. This construct lacks a promoter, instead has a unique 8 bp SwaI endonuclease restriction site to allow for easy 'drop-in' of desired sequence upstream of the fusion reporter.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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