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URL: http://www.addgene.org/21375
Proper Citation: RRID:Addgene_21375
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:8713; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published. NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.
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Source: Addgene
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