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URL: http://www.addgene.org/210788
Proper Citation: RRID:Addgene_210788
Insert Name: Defective hygromycin resistant gene
Organism: Synthetic
Bacterial Resistance: Bleocin (Zeocin)
Defining Citation: PMID:34235660
Vector Backbone Description: Backbone Marker:Dirk Schnappinger; Backbone Size:3592; Vector Backbone:pDE43-MCZ; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: Defective hygromycin-resistant gene was generated by overlap PCR and cloned into the PvuI-HindIII sites of pDE43-MCZ, a mycobacterial phage L5 integrating vector.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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