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URL: http://www.addgene.org/208053
Proper Citation: RRID:Addgene_208053
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:Merck-Millipore (Novagen); Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: We optimized nucleic acid sequence of Twin Strep, eliminated tandem repeats, for better oligo assembly. The nucleic acids are different from that of IBA lifescience, amino acids are the same. The Twin Strep tag was synthesized by oligo assembly using following oligos: # Sequence 5'-3' bp pET28-TwStrp.o1: CGACAAGCTTTCCGGAGGGAGCGCTTGGAGCCACCCGCAGTTCGAA 46 pET28-TwStrp.o2: CGATCCACCGCCAGAACCTCCACCTTTTTCGAACTGCGGGTGGCTC 46 TwStrp.opt.o3: AGGTTCTGGCGGTGGATCGGGAGGTTCAGCGTGGTCTCACCCCCAA 46 TwStrp.opt.o4: GGTGCTCGAGCTATTACTTCTCAAATTGGGGGTGAGACCACGCT 44
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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