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URL: http://www.addgene.org/20792
Proper Citation: RRID:Addgene_20792
Insert Name: mir-124
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19240133
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Comments: 5′-RACE (GenRacer kit, Invitrogen) identified a transcriptional start of the mir-124-5 primary transcript, which is located 1.1 kb from the mir-124-5 hairpin. A 5.6-kb fragment upstream of the mir-124-5 transcriptional start was amplified from genomic DNA using primers 1 and 2 (5'-TGCTTTGAGGCACGGTTATGGTCCC-3' and 5'-CTCGGGCAGCGGCTTTTTGGTCCTG-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
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Source: Addgene
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