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URL: http://www.addgene.org/20185
Proper Citation: RRID:Addgene_20185
Insert Name: Notch 3 Intracellular Domain
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16365048
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3 (5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
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Source: Addgene
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