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URL: http://www.addgene.org/19818
Proper Citation: RRID:Addgene_19818
Insert Name: empty vector
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18840295
Vector Backbone Description: Backbone Size:8200; Vector Backbone:pZ/AP; Vector Types:Mammalian Expression, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: The lacZ and the human placental alkaline phosphatase (AP) genes in the pZ/AP vector were replaced with EGFP, synthesized by PCR from pEGFP-N1 (Clontech), and RFP, synthesized by PCR from pDsRed2-C1 (Clontech), respectively. One cryptic start codon at the 5'UTR of the original AP gene that was not in frame was eliminated. These modifications produced a conditional vector for miRNA expression, pCAG-EGFP/RFP. A synthetic artificial intron was designed to contain typical intron splicing regulatory elements. This intron was placed 10 nt-downstream of the stop codon of the RFP gene to avoid NMD.
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Source: Addgene
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