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URL: http://www.addgene.org/19330
Proper Citation: RRID:Addgene_19330
Insert Name: ttTi5605 targeting region
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18953339
Vector Backbone Description: Backbone Size:2334; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org