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URL: http://www.addgene.org/187656
Proper Citation: RRID:Addgene_187656
Insert Name: Nsp15
Organism: Severe acute respiratory syndrome coronavirus 2
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36630960
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15 (amino acid residues 6453-6798 of YP_009724389). Used for the determination of the atomic-resolution (2.6 angstrom) structure of the room temperature form of SARS-CoV-2 NendoU by serial femtosecond crystallography at an X-ray free-electron laser (XFEL), Protein Data Bank accession number 7K9P, Jernigan et al 2023 Structure 31:1-14, https://doi.org/10.1016/j.str.2022.12.009, PMID 36630960. Confers ampicillin-resistance. Backbone vector is pET-11b from GenScript. Construct design by Debra T. Hansen.
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Source: Addgene
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