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URL: http://www.addgene.org/179289
Proper Citation: RRID:Addgene_179289
Insert Name: Syx
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35639414
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5523; Vector Backbone:pET GFP LIC (2GFP-T) Addgene # 29716; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Protein sequence is MKSS + HHHHHH + GSS + GFP (aa 3-238 of AGM20710.1 with 4 residue differences) + GIE + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + N + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1). Confers ampicillin-resistance. Backbone vector is pET GFP LIC (2GFP-T) Addgene # 29716. Construct design by Ryan Boyd and Debra T. Hansen. Cloning by Felicia M. Craciunescu. Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
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Source: Addgene