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Plasmid Name
pGFP-HsSyx3
RRID:Addgene_179289 RRID Copied  
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RRID:Addgene_179289
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Plasmid Information

URL: http://www.addgene.org/179289

Proper Citation: RRID:Addgene_179289

Insert Name: Syx

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:35639414

Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5523; Vector Backbone:pET GFP LIC (2GFP-T) Addgene # 29716; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Protein sequence is MKSS + HHHHHH + GSS + GFP (aa 3-238 of AGM20710.1 with 4 residue differences) + GIE + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + N + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1). Confers ampicillin-resistance. Backbone vector is pET GFP LIC (2GFP-T) Addgene # 29716. Construct design by Ryan Boyd and Debra T. Hansen. Cloning by Felicia M. Craciunescu. Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821

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Data and Source Information

Source: Addgene