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URL: http://www.addgene.org/17860
Proper Citation: RRID:Addgene_17860
Insert Name: FLIPrbs
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14550551
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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