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URL: http://www.addgene.org/177302
Proper Citation: RRID:Addgene_177302
Insert Name: pe-h
Organism: Pseudomonas aestusnigri
Bacterial Resistance: Kanamycin
Defining Citation: PMID:37333445
Vector Backbone Description: Backbone Size:15436; Vector Backbone:pYTSK01K_0G7; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, Tn7 genomic integration; Bacterial Resistance:Kanamycin
Comments: Part of the pYT vector series which facilitates chromosomal integration in attTn7-site with selection on gentamicin and transcriptional reporter PE-H (https://www.uniprot.org/uniprot/A0A1H6ABJ3; methods: Impranil-assay). Target expression cassettes can be inserted at the I-SceI site.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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