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Plasmid Name
pYTRW20K_0Ti1
RRID:Addgene_177289 RRID Copied  
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RRID:Addgene_177289
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Plasmid Information

URL: http://www.addgene.org/177289

Proper Citation: RRID:Addgene_177289

Insert Name: Ptet-tetA(C)

Organism: Synthetic

Bacterial Resistance: Kanamycin

Defining Citation: PMID:37333445

Vector Backbone Description: Backbone Size:9145; Vector Backbone:yTREX backbone; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, rrn genomic integration; Bacterial Resistance:Kanamycin

Comments: Part of the pYT vector series which enables chromosomal interposon integration via HR in synthetic rrn "landing pads" within 16S rRNA genes of equipped strains. The vector carries the "empty" YT_core with specific cloning slots separated by designated upstream and downstream sequences for integron customization, with TcR marker. Target genes can be inserted at the I-SceI site; additional promoter or reporter elements may be integrated at further positions. The vector backbone, based on the yTREX vector, is equipped with the bacterial pMB1 ori, aphII-encoded KmR and mob gene from pBBR1 (promoter and oriT region) and with yeast replication elements CEN4-ARS1 and URA3 marker. Note: vector pYTRW0268K_0Ti1 also has SacB for counter selection.

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Data and Source Information

Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org