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Plasmid Name
Venus-79aa-ActA-pEGFP-C1
RRID:Addgene_166763 RRID Copied  
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RRID:Addgene_166763
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Plasmid Information

URL: http://www.addgene.org/166763

Proper Citation: RRID:Addgene_166763

Insert Name: ActA tail anchor (Listeria monocytogenes)

Organism: Synthetic

Bacterial Resistance: Kanamycin

Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Comments: "SGLRSRGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDAR GSGLRSRAQASNSAVDARV" amino acid targeting sequence, which we refer to as "ActA", for the mitochondrial outer membrane. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "79aa" indicates the 79 amino acid flexible linker region between Venus and the ActA targeting signal.

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Ratings and Alerts

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Data and Source Information

Source: Addgene