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URL: http://www.addgene.org/166735
Proper Citation: RRID:Addgene_166735
Insert Name: BimEL
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:30860026
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
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Source: Addgene