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URL: http://www.addgene.org/16666
Proper Citation: RRID:Addgene_16666
Insert Name: mTn7::MCS
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16646962
Vector Backbone Description: Backbone Size:12549; Vector Backbone:pGRG36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
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Source: Addgene
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