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URL: http://www.addgene.org/166079
Proper Citation: RRID:Addgene_166079
Insert Name: Promoter of RPL22A
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:263006..263025 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome. Addgene QC identified discrepancies compared to the theoretical sequence. The depositing lab confirms the functional activity of the construct despite these differences.
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Source: Addgene
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