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URL: http://www.addgene.org/16573
Proper Citation: RRID:Addgene_16573
Insert Name: CDK4
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10688915
Vector Backbone Description: Backbone Size:0; Vector Backbone:G1BgSVNA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: The CDK4 ORF was generated by PCR using the expressed sequence tag W77860 as a template and the primers 5'-GCGGATCCGC GGCCGCCTTC CACCATGGCT ACCTCTCGAT CTGAGC-3' and 5'-CGGTCGACTC ACTCCGGATT ACCTTCATC-3'. The resulting product was digested with the enzymes NotI and SalI and inserted into the respective sites of the vector G1BgSVNA (a retroviral vector encoding a hygromycin resistance gene and beta-galactosidase).
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Source: Addgene
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