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URL: http://www.addgene.org/16541
Proper Citation: RRID:Addgene_16541
Insert Name: tTA
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10588737
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMk10-59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: tTA is a a tetracycline-controlled transactivator generated by fusing the tet repressor with the activating domain of virion protein 16 of herpes simplex virus. tTA-IRES-Neo was generated by cloning PCR-amplified tTA cDNA from pUHD15-1 (Gossen, M. & Bujard, H., 1992, PNAS 89, 5547-5551) into the XbaI site of plasmid pMk10-59 (Kobayashi, M et al., 1996, Biotechniques 21, 398-402).
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Source: Addgene