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URL: http://www.addgene.org/16521
Proper Citation: RRID:Addgene_16521
Insert Name: FAST-1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9702198
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.
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Source: Addgene
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