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URL: http://www.addgene.org/163926
Proper Citation: RRID:Addgene_163926
Insert Name: Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
Organism: Drosophila melanogaster
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28395731
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence. pUASTLOTattB vector: Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development 141, 472–480 (2014). vhhGFP4 plasmid: Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K J Mol Biol. 2005 Sep 23; 352(3):597-607. mCherry: Clonetech
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Source: Addgene
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