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URL: http://www.addgene.org/160229
Proper Citation: RRID:Addgene_160229
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Zhang et al. G3. 2014 Oct 15;4(12):2409-18; Backbone Size:2528; Vector Backbone:pXZ13 (Addgene plasmid #60949); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This vector is an improvement upon the published pXZ13 vector for Golden Gate cloning of the donor plasmid in the published article noted below. The vector increases cloning efficiency in two ways: 1) the distance between the Esp3I sites is increased to insure complete cutting at both sites, and 2) lacZ alpha is placed between the two cutting sites to provide Blue/White screening of recombinants. Vector progenitor reference: Zhang X, Koolhaas WH, Schnorrer F. A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3(Bethesda). 2014 Oct 15;4(12):2409-18.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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