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URL: http://www.addgene.org/159446
Proper Citation: RRID:Addgene_159446
Insert Name: BsMDH BmHPS BmPHI (on Addgene plasmid # 129104)
Organism: Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert)
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:33404828
Vector Backbone Description: Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH. This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis.
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Source: Addgene
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