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URL: http://www.addgene.org/154829
Proper Citation: RRID:Addgene_154829
Insert Name: coEPO
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32835482
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:2703; Vector Backbone:pJ204; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This is a donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE) using poly(A)-trap. Promoter 100RPU.2 was initially described in: Brown et al. 2017 "In silico design of context-responsive mammalian promoters with user-defined functionality." DOI: 10.1093/nar/gkx768 A patent application has been filed by third parties on the promoter 100RPU.2 used in this plasmid, permission for deposition of plasmids containing the promoter sequence has been provided by AstraZeneca and The University of Sheffield. The plasmid was assembled by USER cloning.
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No rating or validation information has been found for pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272.
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Source: Addgene