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Plasmid Name
RRID:Addgene_154829 RRID Copied  
PDF Report How to cite
RRID:Addgene_154829
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Plasmid Information

URL: http://www.addgene.org/154829

Proper Citation: RRID:Addgene_154829

Insert Name: coEPO

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:32835482

Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:2703; Vector Backbone:pJ204; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin

Comments: This is a donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE) using poly(A)-trap. Promoter 100RPU.2 was initially described in: Brown et al. 2017 "In silico design of context-responsive mammalian promoters with user-defined functionality." DOI: 10.1093/nar/gkx768 A patent application has been filed by third parties on the promoter 100RPU.2 used in this plasmid, permission for deposition of plasmids containing the promoter sequence has been provided by AstraZeneca and The University of Sheffield. The plasmid was assembled by USER cloning.

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Usage and Citation Metrics

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Ratings and Alerts

No rating or validation information has been found for pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272.

No alerts have been found for pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272.

Data and Source Information

Source: Addgene