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URL: http://www.addgene.org/14853
Proper Citation: RRID:Addgene_14853
Insert Name: p53 R270H
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15607980
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Conditional mutant alleles of p53 were generated by introducing the LoxP-flanked transcriptional STOP cassette into intron 1 of the p53 gene. Site-directed mutagenesis was used to introduce an arg→his mutation at codon 270. Note: This is a large plasmid that can undergo recombination. Bacterial colonies will grow slowly. If there are different colony sizes on a plate, the smaller ones are more likely to contain the correct plasmid.
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Source: Addgene
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