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URL: http://www.addgene.org/14852
Proper Citation: RRID:Addgene_14852
Insert Name: GAL1 promoter TATA-C2
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15166317
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA.
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Source: Addgene
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