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Plasmid Name
p3259 pCMV hUBC9 mt HA
RRID:Addgene_14439 RRID Copied  
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RRID:Addgene_14439
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Plasmid Information

URL: http://www.addgene.org/14439

Proper Citation: RRID:Addgene_14439

Insert Name: UBC9 C93S

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:8668529

Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: A fragment containing the full coding region of hubc9 C93S cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3′-end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.

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Data and Source Information

Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org