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URL: http://www.addgene.org/134312
Proper Citation: RRID:Addgene_134312
Insert Name: cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:31672972
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: pSiMPlc_N should be cotransformed with pSiMPlc_C (or vice versa) in E. Coli to get a reconstituted functional CAT thereby conferring resistance against chloramphenicol. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlc_N + pSiMPlc_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
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Source: Addgene
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