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URL: http://www.addgene.org/125137
Proper Citation: RRID:Addgene_125137
Insert Name: mNeon/mScarletP2A Rac1-N17
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32508591
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-N17 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-N17. Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
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Source: Addgene
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