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URL: http://www.addgene.org/12494
Proper Citation: RRID:Addgene_12494
Insert Name: CRE-M AR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12888538
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: To generate a fusion gene consisting of cre recombinase and the AR LBD, plasmid p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into pCMV-CRE-M(BglII) by digesting two separate aliquots of pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The ligation of these three fragments resulted in the generation of pCMV-CRE-M-AR(wt).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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