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URL: http://www.addgene.org/124889
Proper Citation: RRID:Addgene_124889
Insert Name: Cas9
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26969085
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pMCSV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: Guide RNA to be cloned into BbsI site. Supplied modified golden-gate cloning method is required given that extra BbsI site is located within the 2A sequence. To design guide RNAs: -add ACCG to 5' end of positive strand (positive strand defined as strand which will contact PAM sequence at 3' end) -add AAAC to 5' end of negative strand
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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