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URL: http://www.addgene.org/12457
Proper Citation: RRID:Addgene_12457
Insert Name: mutant TCF/LEF binding sites
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12699626
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4839; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF binding sites that were cloned into the pGL3 vector (Promega), which contains a minimal promoter followed by a firefly luciferase open reading frame. This 134bp fragment was gene synthesized and cloned into the ASP718 and XMA-1 sites of the vector. Note: This plasmid was published as M51 Super 8x FOPFlash, but the plasmid actually contains 6 TCF/LEF sites.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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