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URL: http://www.addgene.org/115971
Proper Citation: RRID:Addgene_115971
Insert Name: X-box binding protein 1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30088945
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6813; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: This sensor leads to the expression of a mNeonGreen fusion protein in the nucleus upon induction of ER-stress. ER-stress kinetic through the IRE1/XBP1 pathway can thus be monitored by recording the green fluorescent signal arising in the nucleus. Designed by Adrien Nougarède.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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