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URL: http://www.addgene.org/114894
Proper Citation: RRID:Addgene_114894
Insert Name: AT1G08590
Organism: Arabidopsis thaliana
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29320478
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SETFQNSEQEILLAFKSDLFDPSNNLQDWKRPENATTFSELVHCHWTGVHCDANGYVAKLLLSNMNLSGNVSDQIQSFPSLQALDLSNNAFESSLPKSLSNLTSLKVIDVSVNSFFGTFPYGLGMATGLTHVNASSNNFSGFLPEDLGNATTLEVLDFRGGYFEGSVPSSFKNLKNLKFLGLSGNNFGGKVPKVIGELSSLETIILGYNGFMGEIPEEFGKLTRLQYLDLAVGNLTGQIPSSLGQLKQLTTVYLYQNRLTGKLPRELGGMTSLVFLDLSDNQITGEIPMEVGELKNLQLLNLMRNQLTGIIPSKIAELPNLEVLELWQNSLMGSLPVHLGKNSPLKWLDVSSNKLSGDIPSGLCYSRNLTKLILFNNSFSGQIPEEIFSCPTLVRVRIQKNHISGSIPAGSGDLPMLQHLELAKNNLTGKIPDDIALSTSLSFIDISFNHLSSLSSSIFSSPNLQTFIASHNNFAGKIPNQIQDRPSLSVLDLSFNHFSGGIPERIASFEKLVSLNLKSNQLVGEIPKALAGMHMLAVLDLSNNSLTGNIPADLGASPTLEMLNVSFNKLDGPIPSNMLFAAIDPKDLVGNNGLCGGVLPPCSKSLALSAKGRNPGR
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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