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URL: http://www.addgene.org/11412
Proper Citation: RRID:Addgene_11412
Insert Name: RNase HII
Organism: M. jannaschii
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10997908
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note that the plasmid pET21a.MJ0135 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
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Source: Addgene
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