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Plasmid Name
pNS20-SpCas9-SNAP
RRID:Addgene_113717 RRID Copied  
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RRID:Addgene_113717
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Plasmid Information

URL: http://www.addgene.org/113717

Proper Citation: RRID:Addgene_113717

Insert Name: Cas9

Organism: Streptococcus pyogenes

Bacterial Resistance: Ampicillin

Defining Citation: PMID:29809142

Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:5938; Vector Backbone:pET His6 MBP N10 TEV LIC cloning vector (2C-T); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin

Comments: Cloning of pNS20-SpCas9-SNAP: pMJ922-SpyCas9-GFP bacterial expression vector was a kind gift from Prof. Martin Jinek. GFP was digested using BamHI and KpnI, and SNAPtag-NLS gBlocks (Integrated DNA Technologies) were integrated using In-Fusion HD Cloning Plus CE (Takara).

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Data and Source Information

Source: Addgene