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URL: http://www.addgene.org/113716
Proper Citation: RRID:Addgene_113716
Insert Name: mutRFP-2A-GFP-IRES-Puro
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29809142
Vector Backbone Description: Backbone Size:8339; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral, Reporter system; Bacterial Resistance:Ampicillin
Comments: Cloning of pNS19-LV-mutRFP-2A-GFP: pEGIP (addgene plasmid #26777) was mutagenized using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to destroy the start codon of eGFP. Next the vector was linearized with BamHI and In-Fusion HD Cloning Plus CE (Takara) was used to insert the mutRFP-2A gBlocks Gene Fragment (Integrated DNA Technologies).
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Source: Addgene
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