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URL: http://www.addgene.org/113630
Proper Citation: RRID:Addgene_113630
Insert Name: mKaxxte2.5
Organism: Other
Bacterial Resistance: Kanamycin
Defining Citation: PMID:30804405
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
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Source: Addgene
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