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URL: http://www.addgene.org/11339
Proper Citation: RRID:Addgene_11339
Insert Name: nud-1
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector. Please note that Addgene's sequencing result differs from GenBank ID NM_067348, which indicates mutations in nud-1 are present at K136R, C220G and E235G. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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