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URL: http://www.addgene.org/113041
Proper Citation: RRID:Addgene_113041
Insert Name: gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30377362
Vector Backbone Description: Vector Backbone:scAAV vector; Vector Types:Mammalian Expression, AAV, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Addgene Next-Generation Sequence analysis identified numerous sequence discrepancies in ITR sequences. Plasmid is expected to function as described in the associated publication: PMID: 30377362
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No rating or validation information has been found for pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold).
No alerts have been found for pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold).
Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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