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URL: http://www.addgene.org/105806
Proper Citation: RRID:Addgene_105806
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29590189
Vector Backbone Description: Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Comments: This vector is a member of the pKK-FRET series which belongs to the pKK vector family. It enables concomitant expression of two genes, which transcription is driven by a tetracycline responsive bidirectional CMV promoter. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101). Negative control for FRET experiments. One protein expressed with Cerulean whereas second protein expressed with non-fluorescent Amber fusion tag. Amber is a mutant of Venus (Y67C) that retains the tertiary structure but has no fluorescent properties and does not absorb light. A no-FRET sample in which both putative interacting proteins are tagged can be superior to a donor-only sample (PMID: 17040988, 22264545). Amber coding sequence was cloned from Addgene ID: 27798.
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Source: Addgene
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