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URL: http://www.addgene.org/105290
Proper Citation: RRID:Addgene_105290
Insert Name: Pak1
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:22547408
Vector Backbone Description: Vector Backbone:pEPet-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Subcloned the sequence encoding amino acids 65-150 (with an additional nucleotide (C) added at the 5' end to keep it in frame) of human Pak1 into the EcoRI-BamH1 sites of pYPet-C1. The insert was from Dr. Natalie Lamarche-Vane (McGill University).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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