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URL: http://www.addgene.org/100633
Proper Citation: RRID:Addgene_100633
Bacterial Resistance: Kanamycin
Defining Citation: PMID:28418644
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 23 of EMMA platform.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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